At Absolute Antibody we have developed a proprietary transient expression system for production of recombinant antibodies and other proteins. We call this the HEXpress system. We utilize a derivative of the human embryonic kidney (HEK293) that has been adapted to suspension expression in serum-free media.
Each component of the HEXpress system has been carefully optimized to increase transient expression yields. We have developed a proprietary vector, transfection reagent, Abfectamine™, a modified serum-free media and a carefully selected cocktail of feeds. Together this makes the HEXpress system one of the highest yielding transient expression systems available from any service provider. Although each antibody has a unique expression profile our yields range between 50 and 500 mg/litre of culture media in as little as 6 days.
Universal antibody vector system
We realize that researchers often want to test their antibody of interest in many different species. Traditionally species and isotype switching by sub-cloning variable domains into alternative vectors has been cumbersome due to incompatible restriction sites and thus the need to generate antibody specific primers and PCR amplify. At Absolute Antibody we have streamlined this process by developing a universal antibody vector, pUV™. This allows rapid and highly efficient switching of variable domains into vectors for antibodies of any species or isotype.