Anti-CD40 [A54 scFv]

Clone Number: A54 scFv
Description: Recombinant monoclonal antibody to CD40. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody A54 scFv.
UniProt Accession Number of Target Protein: P25942
Alternative Name(s) of Target: A54; Tumor necrosis factor receptor superfamily member 5; TNFRSF5; B cell surface antigen CD40; B-cell cell surface antigen CD40; B-cell surface antigen CD40; B cell surface antigen CD40; antigen CD40; CD40 antigen; Bp50; Bp-50; CD40L receptor CDw40 Immunogen: A54 human anti-CD40 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fc? fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
Specificity: A54 binds specifically to the D1 domain of CD40 (and can only bind to the intact i.e. non-truncated version of CD40 and when an AD2 peptide tag has not been added) and can recognise CD40 on a B cell line (BJAB). This scFv binds to CD40 (immobilized by amine coupling) with a KD within the range of 40nM. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation.
Application Notes: A54 is able to block the CD40-CD154 interaction by around 60-80% and can also induce CD40 sinalling in human B cells when at 'unusually high concentrations' (The CD40 receptor - target, tool and technology, Ellmark, 2002). This scFv can also be used for ELISA studies (PMID: 12153507)
Antibody First Published in:
Ellmark et al. In vitro molecular evolution of antibody genes mimicking receptor revision Mol Immunol. 2002 Oct;39(5-6):349-56. PMID:12220892 Note on publication: Describes the creation of an interative library based on 4 similar scFv clones (of which one is A54) using the CDR shiffling principle. Reviews: No reviews available for this antibody yet. Be the first to submit a review at pAbmAbs and enter into their monthly prize draw.