Description: Recombinant monoclonal antibody to CD169. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SER-4 (recombinant version).
Species and Isotype: Rat IgG2a, kappa
Clone Number: SER-4 (recombinant version)
UniProt Accession Number of Target Protein: Q62230
Alternative Name(s) of Target: Sialoadhesin; Sheep erythrocyte receptor; SER; Sialic acid-binding Ig-like lectin 1; Siglec-1
Published Application(s): IHC
Published Species Reactivity: Mouse
Immunogen: Spleen cells from AO rats immunised with thioglycollate-elicted murine peritoneal macrophages (TPM) that had been cutured in mouse serum to induce sheep erythrocyte receptor (SER).
Specificity: This antibody specifically recognize mouse CD169, which is a 180-185-kDa member of the Siglec family of macrophage-specific cell adhesion and endocytic receptors interacting with sialylated glycans. This receptor is expressed on a subset of macrophages that are found with high frequency in the spleen, liver, lymph node, and bone marrow. CD169 can also be detected on monocytes in the bone marrow and periphery.
Antibody First Published in:
Crocker PR, Gordon S. Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody. J Exp Med. 1989 Apr 1;169(4):1333-46. PMID:2926328 Note on publication: Describes the making of SER-4 monoclonal antibody and characterizes the molecular nature and distribution on macrophage of SER with mAb SER-4 - inc data on IHC-Fr, IP and WB assays.

Product Form

Purification: Protein A affinity purified
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.