Description: Recombinant monoclonal antibody to Quadruplex DNA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5C10. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding of Fc Receptor and complement.
Species and Isotype: Mouse IgG1, Fc Silent™, kappa
Clone Number: 5C10
UniProt Accession Number of Target Protein:
Alternative Name(s) of Target: G4 DNA
Published Application(s): ELISA; IHC
Published Species Reactivity: Mammals
Immunogen: Oxy-2 or d(TTTTGGGG)2
Specificity: This antibody binds to quadruplex DNA with a Kd of 24.4 nM. Binds only to the Oxy-2 type.
Application Notes: This antibody binds to quadruplex DNA, which is generated through the association of four guanines bound through Hoogsteen base pairing. G4-forming sequences are associated with immunoglobulin switch regions, promoter sequences, rDNA and telomeric repeats.
Antibody First Published in:
Henderson et al. Detection of G-quadruplex DNA in mammalian cells. Nucleid Acids 2014; 42(2):860-869 PMID:24163102 Note on publication: Describes the generation of several G4-DNA-specific monoclonal antibodies and how these can be used for nuclear staining.

Product Form

Purification: Protein A affinity purified
Supplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.