Ab00551-8.4 Anti-CD6 [OX-52]
Description: Recombinant monoclonal antibody to CD6. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OX-52. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Clone Number: OX-52
UniProt Accession Number of Target Protein:
Published Application(s): FC; IHC-fr; IP
Published Species Reactivity: Rat
Immunogen: This antibody was prepared by immunizing BALB/c mice intraperitoneally with 4x10^7 rat (PVG-RT1c) thoracic duct leucocytes, followed 10 days later by a second injection of 4x 10^7 PVG-RT1c spleen cells.
Specificity: This antibody is specific for rat CD6, which is largely restricted to cells of the T-lymphocyte lineage.
Application Notes: This antibody recognizes rat CD6, which is largely restricted to cells of the T-lymphocyte lineage, and, as a result, this antibody can be used to identify such cells in tissues or cell suspensions (Robinson, 1986). In cell suspensions prepared from lymph node, TDL, thymus and spleen, exclusively T cells are labelled. Using the immunoperoxidase technique on cryostat tissue sections, staining is mainly confined to the T-cell areas of the spleen, lymph nodes and Peyer's patches, as well as all thymocytes. However, occassional positive cells have been observed in the B cell areas of lymphoid organs (Robinson, 1986), and consequently this antibody is used in conjunction with other markers, such as OX-19, to identify T cells.
Antibody First Published in:
Robinson et al. MRC OX-52: A rat T-cell antigen Volume 57, Issue 4, 1986, Pages 527-531 PMID:2937721 Note on publication: Describes the original use of this antibody in preliminary investigations into the nature of its antigen (CD6), and investigates the usefulness of this antibody as a T-cell marker.
Concentration: See vial label.
This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Species and Isotype: Rat IgG2b, Fc Silent™, kappaClone Number: OX-52
UniProt Accession Number of Target Protein:
Published Application(s): FC; IHC-fr; IP
Published Species Reactivity: Rat
Immunogen: This antibody was prepared by immunizing BALB/c mice intraperitoneally with 4x10^7 rat (PVG-RT1c) thoracic duct leucocytes, followed 10 days later by a second injection of 4x 10^7 PVG-RT1c spleen cells.
Specificity: This antibody is specific for rat CD6, which is largely restricted to cells of the T-lymphocyte lineage.
Application Notes: This antibody recognizes rat CD6, which is largely restricted to cells of the T-lymphocyte lineage, and, as a result, this antibody can be used to identify such cells in tissues or cell suspensions (Robinson, 1986). In cell suspensions prepared from lymph node, TDL, thymus and spleen, exclusively T cells are labelled. Using the immunoperoxidase technique on cryostat tissue sections, staining is mainly confined to the T-cell areas of the spleen, lymph nodes and Peyer's patches, as well as all thymocytes. However, occassional positive cells have been observed in the B cell areas of lymphoid organs (Robinson, 1986), and consequently this antibody is used in conjunction with other markers, such as OX-19, to identify T cells.
Antibody First Published in:
Robinson et al. MRC OX-52: A rat T-cell antigen Volume 57, Issue 4, 1986, Pages 527-531 PMID:2937721 Note on publication: Describes the original use of this antibody in preliminary investigations into the nature of its antigen (CD6), and investigates the usefulness of this antibody as a T-cell marker.
Product Form
Purification: Protein A affinity purified Supplied in: Bulk size: PBS only. Standard size: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°CConcentration: See vial label.
Important note – This product is for research use only. It is not intended for use in therapeutic or diagnostic
procedures for humans or animals.
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