Ab00551-8.4 Anti-CD6 [OX-52]
Description: Recombinant monoclonal antibody to CD6. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OX-52. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding of Fc Receptor and complement.
Clone Number: OX-52
UniProt Accession Number of Target Protein:
Published Application(s): FC; IHC; IP; block; depletion; FACS
Published Species Reactivity: Rat
Immunogen: This antibody was prepared by immunizing BALB/c mice intraperitoneally with 4x10^7 rat (PVG-RT1c) thoracic duct leucocytes, followed 10 days later by a second injection of 4x 10^7 PVG-RT1c spleen cells.
Specificity: Rat Thymocytes, T cells, not B, splenic macrophage. CD2 is type I membrane protein involved in triggering of T-cells (cytoplasmic domain is involved in signaling function); expressed on thymocytes, peripheral T cells, dendritic epidermal T cells, macrophages in the spleen, and NK cells.
Application Notes: Ox34 blocks CD48 binding to CD2 causing partial block of T cell activation; OX34 can be used to deplete peripheral T cells (used to prevent allograft rejection in vivo).
Antibody First Published in:
Jefferies et al. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med 162:117 1985 PMID:3159821 Note on publication: Describes the use of OX-34 (among other antibodies) in determining the molecular characteristics of CD4 antigens from macrophages and to determine whether rat macrophages express authentic CD4 antigen; also used to control for the presence of any T cell products in immunoprecipitation from rat macrophage extracts
Supplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.
This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Species and Isotype: Rat IgG2b, Fc Silent™, kappaClone Number: OX-52
UniProt Accession Number of Target Protein:
Published Application(s): FC; IHC; IP; block; depletion; FACS
Published Species Reactivity: Rat
Immunogen: This antibody was prepared by immunizing BALB/c mice intraperitoneally with 4x10^7 rat (PVG-RT1c) thoracic duct leucocytes, followed 10 days later by a second injection of 4x 10^7 PVG-RT1c spleen cells.
Specificity: Rat Thymocytes, T cells, not B, splenic macrophage. CD2 is type I membrane protein involved in triggering of T-cells (cytoplasmic domain is involved in signaling function); expressed on thymocytes, peripheral T cells, dendritic epidermal T cells, macrophages in the spleen, and NK cells.
Application Notes: Ox34 blocks CD48 binding to CD2 causing partial block of T cell activation; OX34 can be used to deplete peripheral T cells (used to prevent allograft rejection in vivo).
Antibody First Published in:
Jefferies et al. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med 162:117 1985 PMID:3159821 Note on publication: Describes the use of OX-34 (among other antibodies) in determining the molecular characteristics of CD4 antigens from macrophages and to determine whether rat macrophages express authentic CD4 antigen; also used to control for the presence of any T cell products in immunoprecipitation from rat macrophage extracts
Product Form
Purification: Protein A affinity purifiedSupplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.
Important note – This product is for research use only. It is not intended for use in therapeutic or diagnostic
procedures for humans or animals.
3629
