Description: Recombinant monoclonal antibody to LMP1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the scFv H3. This is a Fab fragment with a his-tag.
Species and Isotype: Human Fab fragment, His-Tagged, lambda
Clone Number: H3
UniProt Accession Number of Target Protein:
Published Application(s): ELISA; IP; IF; inhibit
Published Species Reactivity: Epstein-Barr virus (EBV)
Immunogen: H3 was selected by its affinity for GST-LMP1 from a human phage display library after 4 rounds of panning.
Specificity: H3 scFv is specific to the LMP1 C terminal region where it binds to CTAR-1 (GST-LMP1[187-242]).  LMP1 is expessed constitutively in many EBV-associated tumours and this protein can cause B lymphocyte and rodent fibroblast transformation, cell apoptosis inhibition by up-regulation of anti-apoptotic proteins, cell surface marker and DNA methyltransferase activty up-regulation, and cell adhesion molecule and cyclin-dependent kinase downregulation. The C-terminal domain of LMP1 is made up of 3 C-terminal activating regions (CTARs) which interact with TNF receptor-associated proteins and TNF receptor-associated death domain protein, which medite activation of NF-kB and AP-1. A LMP1 TAR is also involved in the JAK/STAT pathway.
Application Notes: H3 scFv can reduce LMP1-mediated NF-kappaB activation in HEK293 cells by ~50% as well as inhibit LMP1 functions in epithelial cells - enables the potential use of H3 for attenuating the LMP1 function in LMP1-positive tumors. H3 is also capable of reducing the trans-membrane migration ability of MDCK-LMP1 cells by blocking the CTAR-mediated signaling pathway. H3 can also be used to pull down LMP1 in IPs, and can be used for ELISAs and to visualise LMP1 distibution in IF studies.
Antibody First Published in:
Fang et al. Modulation of Epstein-Barr virus latent membrane protein 1 activity by intrabodies. Intervirology. 2007;50(4):254-63. Epub 2007 Apr 25. PMID:17460414 Note on publication: Describes the generation of and characterization of a number of scFvs specific for different regions of the LMP1 C terminal domain.

Product Form

Purification: Purified by Immobilized Metal Affinity Chromatography
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.