Ab00899-3.0 Anti-CD79b [HM79-16]
Description: Recombinant monoclonal antibody to CD79b. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HM79-16 .
Clone Number: HM79-16
UniProt Accession Number of Target Protein: P15530
Published Application(s): FC; IP; IF; IHC
Published Species Reactivity: Mouse
Immunogen: HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).
Specificity: Reacts with an extracellular epitope of CD79b.
Application Notes: A biotinylated HM79-16 was used and stained with streptavidin-FITC for analysis by FACS to identify variants of B lymphoma cell lines that have lost expression of the mu heavy chain, and for staining using a biotinylated HM79-16 (Condon 2000). HM79-16 has also been used in competitive binding assays to study its effect on the binding of CD79a to CD79b in the context of ameliorating autoimmune disease in mice (Yongmei Li 2008).
Antibody First Published in:
Mariko Koyama et al. CD79α/CD79β heterodimers are expressed on pro-B cell surfaces without associated µ heavy chain International Immunology, Vol. 9, No. 11, pp. 1767–1772 1997 PMID:9418137 Note on publication: Describes the expression of the CD79b and the use of new monoclonal antibodes to detect CD79b on pro-B cell surfaces.
Concentration: See vial label.
This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Species and Isotype: Mouse IgG2b, lambdaClone Number: HM79-16
UniProt Accession Number of Target Protein: P15530
Published Application(s): FC; IP; IF; IHC
Published Species Reactivity: Mouse
Immunogen: HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).
Specificity: Reacts with an extracellular epitope of CD79b.
Application Notes: A biotinylated HM79-16 was used and stained with streptavidin-FITC for analysis by FACS to identify variants of B lymphoma cell lines that have lost expression of the mu heavy chain, and for staining using a biotinylated HM79-16 (Condon 2000). HM79-16 has also been used in competitive binding assays to study its effect on the binding of CD79a to CD79b in the context of ameliorating autoimmune disease in mice (Yongmei Li 2008).
Antibody First Published in:
Mariko Koyama et al. CD79α/CD79β heterodimers are expressed on pro-B cell surfaces without associated µ heavy chain International Immunology, Vol. 9, No. 11, pp. 1767–1772 1997 PMID:9418137 Note on publication: Describes the expression of the CD79b and the use of new monoclonal antibodes to detect CD79b on pro-B cell surfaces.
Product Form
Purification: Protein A affinity purified Supplied in: Bulk size: PBS only. Standard size: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°CConcentration: See vial label.
Important note – This product is for research use only. It is not intended for use in therapeutic or diagnostic
procedures for humans or animals.
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