Description: Recombinant monoclonal antibody to SERT. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 8B6.
Species and Isotype: Rabbit IgG, kappa
Clone Number: 8B6
UniProt Accession Number of Target Protein: P31645
Published Application(s): Crystallisation
Published Species Reactivity: Human
Immunogen: 8B6 was produced by immunising SERT KO mice with residues 73-616 of wildtype human SERT. Hybridomas were generated using established protocols.
Specificity: The 8B6 Fab binds to extracellular loops 2 and 4 of hSERT, as shown by crystallography (Coleman et al, 2016).
Application Notes: The 8B6 Fab fragment was crystallised in complex with human SERT to aid structure determination (Coleman et al, 2016).
Antibody First Published in:
Coleman et al. Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram J Vis Exp. 2016; (117): 54792. PMID:27929454 Note on publication: Describes strategies by which the human serotonin transporter was modified and crystallised. mAb 8B6 was generated using an outlined experimental rationale.

Product Form

Purification: Protein A affinity purified
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.