Ab00943-23.0 Anti-SERT [8B6]
Description: Recombinant monoclonal antibody to SERT. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 8B6.
Clone Number: 8B6
UniProt Accession Number of Target Protein: P31645
Published Application(s): Crystallisation
Published Species Reactivity: Human
Immunogen: 8B6 was produced by immunising SERT KO mice with residues 73-616 of wildtype human SERT. Hybridomas were generated using established protocols.
Specificity: The 8B6 Fab binds to extracellular loops 2 and 4 of hSERT, as shown by crystallography (Coleman et al, 2016).
Application Notes: The 8B6 Fab fragment was crystallised in complex with human SERT to aid structure determination (Coleman et al, 2016).
Antibody First Published in:
Coleman et al. Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram J Vis Exp. 2016; (117): 54792. Note on publication: Describes strategies by which the human serotonin transporter was modified and crystallised. mAb 8B6 was generated using an outlined experimental rationale.
Supplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Species and Isotype: Rabbit IgG, kappaClone Number: 8B6
UniProt Accession Number of Target Protein: P31645
Published Application(s): Crystallisation
Published Species Reactivity: Human
Immunogen: 8B6 was produced by immunising SERT KO mice with residues 73-616 of wildtype human SERT. Hybridomas were generated using established protocols.
Specificity: The 8B6 Fab binds to extracellular loops 2 and 4 of hSERT, as shown by crystallography (Coleman et al, 2016).
Application Notes: The 8B6 Fab fragment was crystallised in complex with human SERT to aid structure determination (Coleman et al, 2016).
Antibody First Published in:
Coleman et al. Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram J Vis Exp. 2016; (117): 54792. Note on publication: Describes strategies by which the human serotonin transporter was modified and crystallised. mAb 8B6 was generated using an outlined experimental rationale.
Product Form
Purification: Protein A affinity purifiedSupplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.
Important note – This product is for research use only. It is not intended for use in therapeutic or diagnostic
procedures for humans or animals.
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