Ab00977-13.12 Anti-Glycoprotein H [MAb 5]
Description: Recombinant monoclonal antibody to Glycoprotein H. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MAb 5.
Clone Number: MAb 5
UniProt Accession Number of Target Protein: P12824
Published Application(s): ELIS; IP; RIA
Published Species Reactivity: Human
Immunogen: The antibody was raised by immunising BALB/c mice against purified HCMV, which expresses gH, in incomplete Freud's adjuvant.
Specificity: The antibody is specific to gH, which has at least two nonoverlapping antigenic sites A and B as well as an overlapping site AB. This antibody binds Site A with an affinity ~5x lower than antibodies for Site B.
Application Notes: The antibody specifically immunoprecipitates the gH protein. Its association constant for gH has been estimated using competetive binding solid-phase RIA.
Antibody First Published in:
Simpson et al. Neutralizing Monoclonal Antibodies That Distinguish Three Antigenic Sites on Human Cytomegalovirus Glycoprotein H Have Conformationally Distinct Binding Sites Journal of Virology, Jan. 1993, p.489-496 Note on publication: Describes the generation of several anti-gH antibodies and mapping of antigenic sites to which they bind.
Supplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Species and Isotype: Human IgG4-P, kappaClone Number: MAb 5
UniProt Accession Number of Target Protein: P12824
Published Application(s): ELIS; IP; RIA
Published Species Reactivity: Human
Immunogen: The antibody was raised by immunising BALB/c mice against purified HCMV, which expresses gH, in incomplete Freud's adjuvant.
Specificity: The antibody is specific to gH, which has at least two nonoverlapping antigenic sites A and B as well as an overlapping site AB. This antibody binds Site A with an affinity ~5x lower than antibodies for Site B.
Application Notes: The antibody specifically immunoprecipitates the gH protein. Its association constant for gH has been estimated using competetive binding solid-phase RIA.
Antibody First Published in:
Simpson et al. Neutralizing Monoclonal Antibodies That Distinguish Three Antigenic Sites on Human Cytomegalovirus Glycoprotein H Have Conformationally Distinct Binding Sites Journal of Virology, Jan. 1993, p.489-496 Note on publication: Describes the generation of several anti-gH antibodies and mapping of antigenic sites to which they bind.
Product Form
Purification: Protein A affinity purifiedSupplied in: PBS with preservative (0.02% Proclin 300)
Storage Recommendation: Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Concentration: See vial label.
Important note – This product is for research use only. It is not intended for use in therapeutic or diagnostic
procedures for humans or animals.
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