Human antibodies from patients are being sequenced at an increasing rate – see Quake et al. for an up to date review of high throughput sequencing of antibodies. Amongst others, we are excited to offer you a range of human-derived antibodies specific to viral pathogens or self-antigen (many in collaboration with Humabs BioMed SA, Bellinzona, Switzerland). Our proprietary cloning platform allows us to produce high quality recombinant versions of these antibodies – we think some will be valuable reagents for research and diagnostics. We offer the original human IgG version as well as a reformatted mouse IgG1 version to suit your experimental requirements- contact email@example.com for bulk custom orders.
Figure 1. Indirect immunofluorescence (IF) staining of live keratinocyte monolayers with pemphigus vulgaris (PV) auto-antibodies
(A) Cryosections of human skin stained with biotinylated DSG3-specific monoclonal antibodies (Ab00153, Ab00155, Ab00158 and Ab00156).
(B) Staining of human epidermis with biotinylated PVB28 (Ab00158) in the presence (with) or absence (w/o) of Ca2+.
(C) Binding of a panel of pemphigus auto-antibodies to DSG3-coated ELISA plates in the presence or absence of EDTA.Antibodies described in:
Corti D, Suguitan AL Jr, Pinna D, Silacci C, Fernandez-Rodriguez BM, Vanzetta F, Santos C, Luke CJ, Torres-Velez FJ, Temperton NJ, Weiss RA, Sallusto F,Subbarao K, Lanzavecchia A. Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccine. J Clin Invest 120, 1663–1673 (2010).
Di Zenzo G, Di Lullo G, Corti D, Calabresi V, Sinistro A, Vanzetta F, Didona B, Cianchini G, Hertl M, Eming R, Amagai M, Ohyama B, Hashimoto T, Sloostra J, Sallusto F, Zambruno G, Lanzavecchia A. Pemphigus autoantibodies generated through somatic mutations target the desmoglein-3 cis-interface. J Clin Invest. 2012 Oct 1;122(10):3781-90.