Frustrated by background staining? Then you’ll love Fc Silent™!
Absolute Antibody’s Fc Silent™ antibodies are specifically designed to reduce non-specific background antibody staining, instantly improving the quality of your experiments.
One of the main causes of no
n-specific background staining is the binding of antibodies to Fcγ receptors (FcγR), making many experiments involving cells expressing FcγRs difficult to interpret.
Fc Silent™ antibodies contain engineered Fc domains with abolished FcγR binding. Fc Silent™ does not affect other properties of the antibody, including their compatibility with secondary antibodies.
Fc Silent™…and that’s it!
As an example, we show how murine macrophages are labelled almost as strongly by a rat IgG2b isotype control as by an anti-F4/80 (Figure 1). Switching to Fc Silent™ antibodies results in reliable data with background staining indistinguishable from a secondary-antibody-only control.
This also shows that Fc Silent™ antibodies are compatible with standard secondary antibodies.
In more detail using SPR (Figure 2), Fc Silent™ shows no detectable interaction with murine FcγRs. You can
find further data on Fc Silent™ here.
Compared to Fc receptor blocking reagents, Fc Silent™ antibodies do not require additional steps in your experiment and are more widely compatible with secondary antibodies than F(ab’)2 and Fab fragments.
Fc Silent™ for Everyone
Since scientists have different requirements, we are offering all of our catalogue antibodies as Fc Silent™, spanning over 5 different species (mouse, rat, human, rabbit, hamster) and every possible isotype…and if you can’t yet find what you’re looking for, let us know and we will make it for you in as little as 2 weeks!
Any questions? Get in touch at firstname.lastname@example.org