Frustrated by background staining? Then you’ll love Fc Silent™!
Absolute Antibody’s Fc Silent™ antibodies are specifically designed to reduce non-specific background antibody staining, instantly improving the quality of your experiments.
One of the main causes of non-specific background staining is the binding of antibodies to Fcγ receptors (FcγR), making many experiments involving cells expressing FcγRs difficult to interpret. Fc Silent™ antibodies contain engineered Fc domains with abolished FcγR binding. Fc Silent™ does not affect other properties of the antibody, including their compatibility with secondary antibodies.
Fc Silent™…and that’s it!
As an example, we show how murine macrophages are labelled almost as strongly by a rat IgG2b isotype control as by an anti-F4/80 (Figure 1). Switching to Fc Silent™ antibodies results in reliable data with background staining indistinguishable from a secondary-antibody-only control.
This also shows that Fc Silent™ antibodies are compatible with standard secondary antibodies.
In more detail using SPR (Figure 2), Fc Silent™ shows no detectable interaction with murine FcγRs. You can find further data on Fc Silent™ here.
Compared to Fc receptor blocking reagents, Fc Silent™ antibodies do not require additional steps in your experiment and are more widely compatible with secondary antibodies than F(ab’)2 and Fab fragments.