Fc Silent™ represents a genetically engineered Fc domain developed at Absolute Antibody for research and assay development use. It contains key point mutations that abrogate binding of Fc receptors (FcγR, FcR) abolishing antibody directed cytotoxicity (ADCC) effector function.
All our antibodies are available as Fc Silent™. Click here to view our catalogue. If you can’t find the antibody you are looking for, get in touch and we will be able to generate it for you in often as little as 2 weeks.
Fc Silent™ Recombinant monoclonal antibodies and Fusion proteins benefit from the long plasma half-life (t1/2) associated with the Fc domain, with none of the cytolytic immune effector mechanisms associated with the wild-type Fc domain. In vitro applications for Fc Silent™ monoclonal antibodies and Fusion proteins include flow cytometryand immunohistochemistry (IHC). When studying immune cells, flow cytometry and IHC data analysis is complicated by non-specific background signal resulting from binding of the Fc domain to Fc receptors.
For example, mouse IgG2a and rat IgG2b isotypes are known to bind strongly to Fc receptors irrespective of the test antibody specificity. Isotype controls are employed to allow the researcher to distinguish between specific and non-specific background signals.
As an example, we show how murine macrophages are labelled almost as strongly by a rat IgG2b isotype control as by an anti-F4/80. Switching to Fc Silent™ antibodies results in reliable data with background staining indistinguishable from a secondary-antibody-only control.
This also shows that Fc Silent™ antibodies are compatible with standard secondary antibodies.
Absolute Antibody offer a diverse set of species specific Fc Silent™ domains (human, mouse, rabbit, rat, hamster) for our monoclonal antibody and Fusion protein catalog and custom service product range.
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View more Fc Silent™ binding data.