United Kingdom (UK)
Recombinant monoclonal antibody to Bax (active monomer). Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 6A7.
UniProt Accession Number of Target Protein: Q07812
Alternative Name(s) of Target: Bcl-2-like protein 4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4
Immunogen: KLH conjugated to peptides corresponding to amino acids 3–16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3–16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12–24 shared by Bax from both species (CGPTSSEQIMKTGA).
Specificity: In the presence of nonionic detergent, the antibody avidly binds the monomeric form of Bax, but not Bax complexed with either Bcl-XL or Bcl-2. [6A7 ] Bax antibody only recognizes conformationally changed active Bax.
Antibody first published in:
Hsu YT, Youle RJ. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 1997 May 23;272(21):13829-34. PMID:9153240
Note on publication:
Describes the making of the species-independent Bax monoclonal antibodies, shows it recognised monomeric form of Bax, and analysed by immunoprecipitation followed by immunoblotting.
Immunohistochemical staining of human lung cancer using anti-Bax antibody Ab00120-23.0 Formalin fixed human lung cancer slices were were stained with a Ab00120-23.0 at 3 µg/ml.