Anti-IL-18 [125-2H]

Description:

Recombinant monoclonal antibody to IL-18. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 125-2H.

Clone Name:

125-2H

Published Application(s):

IP, NTRL, ELISA, IF

Published Species Reactivity:

Human

Products based on this clone:

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Ab00140-1.1 Anti-IL-18 [125-2H]
Human
Mouse IgG1
Purified
In Stock

Ab00140-23.0 Anti-IL-18 [125-2H]
Human
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: Q14116

Alternative Name(s) of Target: Iboctadekin; Interleukin 18 (Interferon-gamma-inducing factor); IGIF; IL1F4; IL-1g
Immunogen: Fusion of splenocytes from mice immunized with human IL-18, with SP2/O mouse myeloma cells.

Specificity: Recognizes the active form of human IL-18.

Antibody first published in: Taniguchi M, Nagaoka K, Kunikata T, Kayano T, Yamauchi H, Nakamura S, Ikeda M, Orita K, Kurimoto M. Characterization of anti-human interleukin-18 (IL-18)/interferon-gamma-inducing factor (IGIF) monoclonal antibodies and their application in the measurement of human IL-18 by ELISA. J Immunol Methods. 1997 Aug 7;206(1-2):107-13. PMID:9328573
Note on publication: Describes the generation of murine monoclonal antibody [125-2H] and its ability to inhibit IL-18-induced IFN-γ production by KG-1 cells.

WB on HeLa cell lysate using Ab00140-23.0. Click on image for more details.

Western Blot using anti-IL18 antibody 125-2H (Ab00140) HeLa cell lysate samples (35µg protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 125-2H (Ab00140-23.0) at 2 µg/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL18 is 22.3kDa. Ab00140-23.0 successfully detected IL18 in HeLa cell lysate samples.

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