United Kingdom (UK)
Recombinant monoclonal antibody to P-glycoprotein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MRK16.
UniProt Accession Number of Target Protein: P08183
Alternative Name(s) of Target: ABCB1; Multidrug resistance protein 1; ATP-binding cassette sub-family B member 1; CD243; MDR1; PGP; PGY1
Immunogen: Drug resistant K-562 cells.
Specificity: Recognizes a discontinuous extracellular epitope on P-glycoprotein.
Antibody first published in:
Hamada H, Tsuruo T. Functional role for the 170- to 180-kDa glycoprotein specific to drug-resistant tumor cells as revealed by monoclonal antibodies. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7785-9. PMID: 2429319
Note on publication:
Describes the generation of the antibody and its ability to modulate drug cytotoxicity in vitro.
Flow-cytometry using the anti-P-glycoprotein antibody MRK16 (Ab00143) HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of MRK16 (Ab00143-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.