- {{heading}}
- Ab00161-7.1 Anti-CD169 [SER-4 (recombinant version)]
- Mouse
- Rat IgG2a
- Purified
- In Stock
- Ab00161-23.0 Anti-CD169 [SER-4 (recombinant version)]
- Mouse
- Rabbit IgG
- Purified
- In Stock
Recombinant monoclonal antibody to CD169. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SER-4 (recombinant version).
UniProt Accession Number of Target Protein: Q62230
Alternative Name(s) of Target: Sialoadhesin; Sheep erythrocyte receptor; SER; Sialic acid-binding Ig-like lectin 1; Siglec-1
Immunogen: Spleen cells from AO rats immunised with thioglycollate-elicted murine peritoneal macrophages (TPM) that had been cutured in mouse serum to induce sheep erythrocyte receptor (SER).
Specificity: This antibody specifically recognize mouse CD169, which is a 180-185-kDa member of the Siglec family of macrophage-specific cell adhesion and endocytic receptors interacting with sialylated glycans. This receptor is expressed on a subset of macrophages that are found with high frequency in the spleen, liver, lymph node, and bone marrow. CD169 can also be detected on monocytes in the bone marrow and periphery.
Antibody first published in:
Crocker PR, Gordon S. Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody. J Exp Med. 1989 Apr 1;169(4):1333-46. PMID:2926328
Note on publication:
Describes the making of SER-4 monoclonal antibody and characterizes the molecular nature and distribution on macrophage of SER with mAb SER-4 - inc data on IHC-Fr, IP and WB assays.
Immunofluorescence staining of RAW264.7 cells with anti-CD169 (Ab00161) SER-4 (recombinant version) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells stained with the chimeric rabbit IgG version of SER-4 (recombinant version) (Ab00161-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00161-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-CD169 antibody SER-4 (recombinant version) (Ab00161). Paraformaldehyde fixed RAW264.7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SER-4 (recombinant version) (Ab00161-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of RAW264.7 cells with anti-CD169 (Ab00161) SER-4 (recombinant version) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells stained with the chimeric rabbit IgG version of SER-4 (recombinant version) (Ab00161-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00161-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.