United Kingdom (UK)
Recombinant monoclonal antibody to CD2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma YTH 655.
UniProt Accession Number of Target Protein: P06729
Alternative Name(s) of Target: LFA-2; T-cell surface antigen CD2; Erythrocyte receptor; LFA-3 receptor; Rosette receptor; T-cell surface antigen T11/Leu-5
Immunogen: This antibody was raised against CD2 expressing cells.
Specificity: This antibody specifically recognizes human CD2, a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells.
Antibody first published in:
Qin S, Cobbold SP, Pope H, Elliott J, Kioussis D, Davies J, Waldmann H. "Infectious" transplantation tolerance. Science. 1993 Feb 12;259(5097):974-7. PMID:8094901
Note on publication:
Describes it's use in vivo (human CD2+/CBA transgenic mice) - including Flow-Cytometry data.
Western Blot using anti-CD2 antibody YTH 655 (Ab00164) Human tonsil lysate samples (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of YTH 655 (Ab00164-23.0) at 1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD2 is 39.5kDa, but this protein is glycosylated at several residues. Ab00164-23.0 successfully detected CD2 in human tonsil lysate.
Immunofluorescence staining of fixed Molt4 cells with anti-CD2 antibody YTH 655 (Ab00164) Immunofluorescence analysis of paraformaldehyde fixed Molt4 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YTH 655 (Ab00164-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane, and some cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00164-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (Ab102-23.0) followed by Alexa Fluor® 488 secondary antibody.