United Kingdom (UK)
Recombinant monoclonal antibody to Notch 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display derived scfv E6.
UniProt Accession Number of Target Protein: P46531 (human); Q01705 (mouse)
Alternative Name(s) of Target: Tan1; Notch1; Mis6; N1; lin-12; NOTCH1; TAN1; hN1
Immunogen: This antibody was selected against a fusion protein consisting of EGF domains 1–12 of murine Notch1 fused to a human Fc domain and binds to to the NRR of mouse Notch1.
Specificity: An engineered ScFv fragment antibody that binds to both mouse mNRR1 and human hNRR1 but not NRR2.
Antibody first published in:
Falk R, Falk A, Dyson MR, Melidoni AN, Parthiban K, Young JL, Roake W, McCafferty J. Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods. 2012 Sep;58(1):69-78. PMID:22842086
Note on publication:
Describes the generation of Notch receptor blocking antibodies using Phage Display.
Flow-cytometry using the anti-Notch 1 antibody E6 (Ab00175) Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (Ab00175-23.0, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.
Immunofluoresence staining of fixed HeLa cells with anti-Notch 1 antibody E6 (Ab00175) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of E6 (Ab00175-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00175-23.0, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-Notch 1 antibody E6 (Ab00175) Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of E6 (Ab00175-23.0, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.