United Kingdom (UK)
Recombinant monoclonal antibody to CD45R. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma YTH 80.103.
UniProt Accession Number of Target Protein: P08575
Alternative Name(s) of Target: Ly-5; Lyt-4; T200; CD45 R; L-CA; Leukocyte common antigen; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200; YTH80; YTH-80
Immunogen: Human T lymphocytes.
Specificity: Recognises a sialic acid dependent carbohydrate epitope associated with the C exon on all of the CD45 four isoforms.
Antibody first published in:
Hale et al. Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol. 1985 May;134(5):3056-61. PMID:3980990
Note on publication:
Describes the characterisation of the complement fixing ability of the original rat IgG2b version of this antibody.
Flow-cytometry using anti-CD45R antibody YTH 80.103 (Ab00196) Human leukocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of YTH 80.103 (Ab00196-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.