Anti-Phosphotyrosine [PY20] Bulk size

Description: Recombinant monoclonal antibody to Phosphotyrosine. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma PY20. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Isotype and Format: Mouse IgG1, Fc Silent™, kappa
Clone Number: PY20
Published Application(s): WB; IF; IHC-P; IHC-FoFr; IP
Published Species Reactivity: Species independent
Immunogen: Phosphotyrosine conjugated to Keyhole limpet hemocyanin (KLH).
Specificity: PY20 recognizes phospho-tyrosine and phosphotyrosine containing proteins with a reported Kd of 500 nM for the original clone.Whilst phospho-Phenylalanine (Kd = 4 uM M), phospho-Serine (>100 mM) & phospho-Threonine (100 mM) are also detected, the differences in affinity make this antibody primarily phospho-tyrosine specific.

Antibody First Published in:
Glenney et al. J Immunol Methods. PMID:2452204 Note on publication: Describes generation of anti-phosphotyrosine antibodies, including PY20

Product Form

This is a Bulk size product. Purified antibody in bulk size.
Purification: Protein A affinity purified
Supplied in: PBS only.
Storage Recommendation: Store at 4°C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00294-1.4-BT

Mouse IgG1

In stock
Standard size 200 µg
Custom conjugation:
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