Anti-IL-18 [SAIC-24B-4]

Description:

Recombinant monoclonal antibody to IL-18. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-24B-4.

Clone Name:

SAIC-24B-4

Published Application(s):

immunoMRM

Published Species Reactivity:

Human

Products based on this clone:

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Ab00316-23.0 Anti-IL-18 [SAIC-24B-4]
Human
Rabbit IgG
Purified
In Stock

Ab00316-1.1 Anti-IL-18 [SAIC-24B-4]
Human
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: Q14116

Alternative Name(s) of Target: Interleukin-18, IGIF, IL1F4
Immunogen: The KLH conjugated peptide "ISTLSCENK" derived from IL-18, an augmentor of natural killer cell activity in the spleen and stimulator of interferon gamma production in Th1 cells.

Specificity: Recognises human IL18.

Application Notes: Original data characterizing this antibody may be found here .

Antibody first published in: Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.

Flow-cytometry on human peripheral bloood monocytes using Ab00316-23.0. Click on image for more details.

Flow-cytometry using the anti-IL-18 antibody SAIC-24B-4 (Ab00316) Human peripheral blood monocytes (PBMC’s) were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of SAIC-24B-4 (Ab00316-23.0, blue line) at a dilution of 1:100 for 1h at RT. An additional population of PBMC’s stimulated with PMA and Ionomycin in the presence of Monensin for 4 hours were stained in a similar manner (purple line). After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

WB on human PBM lysate using Ab00316-23.0. Click on image for more details.

Western Blot using anti-IL-18 antibody SAIC-24B-4 (Ab00316) Untreated human peripheral blood monocyte (PBM; A) and PMA/lonomycin-treated PBM lysates (35µg protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of SAIC-24B-4 (Ab00316-23.0) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL-18 is 22.3kDa. Ab00316-23.0 successfully detected IL-18 in untreated human peripheral blood monocyte samples.

Flow-cytometry on human peripheral bloood monocytes using Ab00316-23.0. Click on image for more details.

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