Anti-LGALS1 [SAIC-25B-112]

Description:

Recombinant monoclonal antibody to LGALS1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-25B-112.

Clone Name:

SAIC-25B-112

Published Application(s):

immunoMRM

Published Species Reactivity:

Human

Products based on this clone:

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Ab00317-23.0 Anti-LGALS1 [SAIC-25B-112]
Human
Rabbit IgG
Purified
In Stock

Ab00317-1.1 Anti-LGALS1 [SAIC-25B-112]
Human
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: P09382

Alternative Name(s) of Target: Galectin-1
Immunogen: The KLH-conjugated peptide "SFVLNLGK" derived from Galectin 1. Galectin 1 binds beta-galactoside and other complex carbohydrates and may regulate apoptosis, cell proliferation and cell differentiation.

Specificity: Recognises human LGALS1.

Application Notes: Original data characterizing this antibody may be found here .

Antibody first published in: Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.

Flow cytometry analysis of HeLa cells using Ab00317-23.0. Click on image for more details.

Flow cytometry using the Anti-LGALS1 antibody SAIC-25B-112 (Ab00317). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-25B-112 (Ab00317-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western blot on U251 cell lysate using Ab00317-23.0. Click on image for more details.

Western Blot using anti-LGALS1 antibody SAIC-25B-112 (Ab00317). U251 cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-25B-112 (Ab00317-23.0) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

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