Anti-RAD23B [SAIC-28B-8]

Description:

Recombinant monoclonal antibody to RAD23B. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-28B-8.

Clone Name:

SAIC-28B-8

Published Application(s):

immunoMRM

Published Species Reactivity:

Human

Products based on this clone:

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Ab00321-23.0 Anti-RAD23B [SAIC-28B-8]
Human
Rabbit IgG
Purified
In Stock

Ab00321-1.1 Anti-RAD23B [SAIC-28B-8]
Human
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: P54727

Alternative Name(s) of Target: HHR23B; HR23B; P58; RAD23 homolog B; nucleotide excision repair protein; UV excision repair protein RAD23 homolog B
Immunogen: Peptide "IDIDPEETVK", derived from the UV excision repair protein RAD23 homolog B, conjugated to KLH.

Specificity: Recognises human RAD23B.

Application Notes: Original data characterizing this antibody may be found here .

Antibody first published in: Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.

Flow cytometry analysis of HeLa cells using Ab00321-23.0. Click on image for more details.

Flow cytometry using the Anti-RAD23B antibody SAIC-28B-8 (Ab00321). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-28B-8 (Ab00321-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western blot on nuclear lysate of MCF7 cells using Ab00321-23.0. Click on image for more details.

Western Blot using anti-RAD23B antibody SAIC-28B-8 (Ab00321). Nuclear lysate of MCF7 cells (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-28B-8 (Ab00321-23.0) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

IF staining of HeLa cells using Ab00321-23.0. Click on image for more details.

Immunofluorescence staining of HeLa cells with anti-RAD23B (Ab00321) SAIC-28B-8 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-28B-8 (Ab00321-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00321-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

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