United Kingdom (UK)
Recombinant monoclonal antibody to N-acyl homoserine lactones. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RS2-1G9.
Alternative Name(s) of Target: HSL;AHL
Immunogen: The 4-methoxyphenyl amide AHL analog.
Specificity: Binds 3-oxo-C12-HSL with a Kd of 150 nM
Application Notes: This antibody, originally raised in mice, binds to several homoserine lactones from P. aeruginosa. Homoserine lactones (HSL) are signalling molecules in bacteria used for quorum sensing, required for successful estabilshment of infecion. RS2-1G9 interferes with quorum sensing and was shown to protect murine bone marrow derived macrophages, from the cytotoxic P. aeruginosa quorum sensing signalling molecule N-3-oxo-dodecanoyl-homoserine lactone (PMID: 18304641).
Antibody first published in:
Kaufmann et al. 2006 Antibody Interference with N-Acyl Hosoerine Lacone-Mediated Bacterial Quorum Sensing. Journal of the American Chemical Society. 2006; 128 (9): 2802-2803 PMID:16506750
Note on publication:
Describes the design of HSL-analogous haptens used for the immunization of mice for hybridoma production of the 1G9 antibody as well as charcterization of the inhibibition of quorum sensing by the produced antibodies.