Anti-Quadruplex DNA [1H6] Standard size

Description: Recombinant monoclonal antibody to Quadruplex DNA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1H6.
Isotype and Format: Mouse IgG1, kappa
Clone Number: 1H6
Published Application(s): ELISA; IHC; IF
Published Species Reactivity: Mammals
Immunogen: Oxy-2 or d(TTTTGGGG)2.
Specificity: The antibody binds to quadruplex DNA with a Kd of 0.33 nM. Binds to both types of quadruplex DNA, Oxy-2 (d(TTTTGGGG)2) and Ver-3 (d(TGGGGG(TTAGGG)2T).
Application Notes: This antibody binds to quadruplex DNA, which is generated through the association of four guanines bound through Hoogsteen base pairing. G4-forming sequences are associated with immunoglobulin switch regions, promoter sequences, rDNA and telomeric repeats. Ab00389-1.1 was used by Hansel-Hertsch et al. 2016 (PMID: 27618450) in immunofluorescence staining (in comparison to the BG4 anti-G quadruplex antibody, c.f. Ab00174).
Antibody First Published in:
Henderson et al. Nucleid Acids 2014; 42(2):860-869 PMID:24163102 Note on publication: Describes the generation of several G4-DNA-specific monoclonal antibodies and how these can be used for nuclear staining.

Product Form

This is a Standard size product. Purified antibody.
Purification: Protein A affinity purified
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00389-1.1

Mouse IgG1

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Standard size 200 µg
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