United Kingdom (UK)
Recombinant monoclonal antibody to Quadruplex DNA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1H6.
Alternative Name(s) of Target: G4 DNA
Immunogen: Oxy-2 or d(TTTTGGGG)2.
Specificity: The antibody binds to quadruplex DNA with a Kd of 0.33 nM. Binds to both types of quadruplex DNA, Oxy-2 (d(TTTTGGGG)2) and Ver-3 (d(TGGGGG(TTAGGG)2T).
Application Notes: This antibody binds to quadruplex DNA, which is generated through the association of four guanines bound through Hoogsteen base pairing. G4-forming sequences are associated with immunoglobulin switch regions, promoter sequences, rDNA and telomeric repeats. Ab00389-1.1 was used by Hansel-Hertsch et al. 2016 (PMID: 27618450) in immunofluorescence staining (in comparison to the BG4 anti-G quadruplex antibody, c.f. Ab00174).
Antibody first published in:
Henderson et al. Detection of G-quadruplex DNA in mammalian cells. Nucleid Acids 2014; 42(2):860-869 PMID:24163102
Note on publication:
Describes the generation of several G4-DNA-specific monoclonal antibodies and how these can be used for nuclear staining.