Anti-EGFR [L3 11D] Bulk size

Description: Recombinant monoclonal antibody to EGFR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the scFv L3 11D.
Isotype and Format: Mouse IgG2a, kappa
Clone Number: L3 11D
Published Application(s): ELISA; WB; SDS-PAGE; FC
Published Species Reactivity: Human
Immunogen: Human EGFR.
Specificity: The antibody binds to EGFR with an affinity (Kd) of 0.21 nM.
Application Notes: The antibody binds specifically to EGFR, a cell-surface receptor which is part of the ErbB family of receptors. The EGFR is a receptor tyrosine kinase, which, upon activation by its ligands, such as epidermal growth factor and transforming growth factor alpha, triggers signal transduction pathways that increase cell migration, adhesion and proliferation. Mutations that lead to the overexpression of EGFR are associated with the development of cancer. The antibody was prepared from phage antibodies isolated from the lymph node library.
Antibody First Published in:
Kettleborough et al. Eur. J. Immunol. 24 (4), 952-958 (1994) PMID:8149964 Note on publication: Describes the phage-antibody technology, which is used to produce three phage-antibody libraries from which mouse anti-EGFR scFv antibodies are generated. The libraries are prepared from the spleen, lymph node and in vitro cells of an immunized mouse. The antibodies produced are compared with a mouse mAb, 425, isolated by standard hybridoma technology.

Product Form

This is a Bulk size product. Purified antibody in bulk size.
Purification: Protein A affinity purified
Supplied in: PBS only.
Storage Recommendation: Store at 4°C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00455-2.0-BT

Mouse IgG2a

In stock
Standard size 200 µg
Custom conjugation:
Clear selection