United Kingdom (UK)
Recombinant monoclonal antibody to CD30. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the scFv Ki-4.
UniProt Accession Number of Target Protein: P28908
Alternative Name(s) of Target: TNFRSF8; Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30
Immunogen: Human CD30.
Specificity: The antibody binds to CD30.
Application Notes: The antibody binds specifically to CD30, a cell membrane protein which belongs to the tumour necrosis factor receptor family. Incubation of CD30 positive L540 cells with Ki-4 antibody completely inhibits release of soluble CD30 (in contrast to Ki-3, Ab00474). CD30 is expressed on activated lymphocytes, and binding by its ligands TRAF2 and TRAF5 results in the activation of NF-kappaB, thereby regulating gene expression. CD30 is also associated with Hodgkin Lymphoma, and its expression acts as a tumour marker. Targeting the CD30 antigen with specific monoclonal antibodies is a possible therapy for Hodgkin's lymphoma.
Antibody first published in:
Horn-Lohrens O, Tiemann M, Lange H, Kobarg J, Hafner M, Hansen H, Sterry W, Parwaresch RM, Lemke H. Shedding of the soluble form of CD30 from the Hodgkin-analogous cell line L540 is strongly inhibited by a new CD30-specific antibody (Ki-4). Int J Cancer. 1995 Feb 8;60(4):539-44. PMID:7530238
Note on publication:
Describes the role of CD30 in the detection of Hodgkin lymphoma, and the production of monoclonal antibodies to inhibit the formation of soluble CD30 and thereby allow the production of immunotoxins.
Flow-cytometry using anti-CD30 antibody Ki-4 (Ab00475) Human lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of Ki-4 (Ab00475-23.0) before (B) or after anti-CD3/28 activation (C) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.