United Kingdom (UK)
Recombinant monoclonal antibody to LeX. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 291-2G3-A.
Alternative Name(s) of Target: Sialyl Lewis X; SLeX; Stage-specific embryonic antigen 1; SSEA-1; CD15
Immunogen: This antibody was obtained from mice infected with schistosomes.
Specificity: The antibody binds to Lewis X trisaccharide, a carbohydrade pivotal in mediating specific cell-cell interactions, with an affinity of 11 μM.
Application Notes: The antibody binds specifically to Lewis X, a tetrasaccharide carbohydrate blood-group antigen involved in cell-to-cell adhesion. The antigen is involved in human fertilisation, as it is present on the terminus of N- and O-glycans of the zona pellucida of egg cells, which are recognised by sperm cells This interaction allows fusion and fertilisation to occur. LeX also plays a role in leukocyte tethering as they travel through the bloodstream as it can interact with L-selectin, P-selectin and E-selectin. LeX is also present on neutrophils, mediating phagocytosis and chemotaxis, and is expressed in patients with Hodgkin disease, B-cell chronic lymphocytic leukemia, acute lymphoblastic leukemias and acute nonlymphocytic leukemia. It is also expressed as SSEA-1 on mouse embryonic stem cells. LeX also plays a role in human resistance to the parasitic disease schistosomiasis.
Antibody first published in:
van Roon AM, Pannu NS, Hokke CH, Deelder AM, Abrahams JP. Crystallization and preliminary X-ray analysis of an anti-Lewis X Fab fragment with and without its Lewis X antigen. Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1306-9. Epub 2003 Jun 27. PMID:12832795
Note on publication:
Describes the production of the Fab fragment of the monoclonal antibody 291-2G3-A, used to crystallise the structure of Lewis X.