Anti-Prion [3F4]

Description:

Recombinant monoclonal antibody to Prion. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3F4.

Clone Name:

3F4

Published Application(s):

IP, WB, ELISA, IHC

Published Species Reactivity:

Hamster, Human

Products based on this clone:

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Ab00496-1.1 Anti-Prion [3F4]
Hamster, Human
Mouse IgG1
Purified
In Stock

Ab00496-10.0 Anti-Prion [3F4]
Hamster, Human
Human IgG1
Purified
In Stock

Ab00496-10.6 Anti-Prion [3F4]
Hamster, Human
Human Fab fragment
His-Tagged
Purified
Ships in 4-5 weeks

Ab00496-10.7 Anti-Prion [3F4]
Hamster, Human
Human F(ab)2
AbFab2™ His-Tagged
Purified
Ships in 4-5 weeks

Ab00496-22.0 Anti-Prion [3F4]
Hamster, Human
Hamster (Armenian) IgG
Purified
Ships in 4-5 weeks

Alternative Name(s) of Target: Major prion protein; p27-30; CD30 antigen; prion protein PrP; prion-related protein
Immunogen: Hamster 263K PrPs.

Application Notes: The antibody binds specifically to prion, a misfolded protein that has the ability to enter healthy organisms and cause other misfolding of other proteins into their prion form. This plays an essential role in the pathogenesis of certain neurodegenerative disorders such as Creutzfeldt-Jakob disease (CJD), Gerstmann-Staussler syndrome (GSS) and bovine spongiform encephalopathy (BSE). These disorders are characterised by an accumulation of prion proteins in the brain, which have a disrupted secondary structure.

Antibody first published in: Kascsak RJ, Rubenstein R, Merz PA, Tonna-DeMasi M, Fersko R, Carp RI, Wisniewski HM, Diringer H. Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins. J Virol. 1987 Dec;61(12):3688-93. PMID:2446004
Note on publication: Describes the generation a species-specific monoclonal antibody directed against protease-resistant proteins (PrPs) in order to study the relationship of PrPs to their host precursor protein and to scrapie-associated fibril proteins.

IF staining of U251 cells using Ab00496-1.1. Click on image for more details.

Immunofluorescence staining of fixed U251 cells with anti-Prion antibody 3F4 (Ab00496) Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 3F4 (Ab00496-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00496-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.

WB on human brain cerebral cortex, cerebellum, and hippocampus lysates using Ab00496-1.1. Click on image for more details.

Western Blot using anti-major Prion protein antibody 3F4 (Ab00496) Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of 3F4 (Ab00496-1.1). Cerebral cortex and hippocampus samples were probed using 0.001 µg/ml of Ab00496-1.1, and cerebellum samples with 0.003 µg/ml of Ab00496-1.1, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. Ab00496-1.1 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.

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