United Kingdom (UK)
Recombinant monoclonal antibody to Methylene diphenyl diisocyanate. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AH6.
Alternative Name(s) of Target: MDI
Immunogen: Mouse serum protein conjugated with MDI.
Application Notes: The antibody binds specifically to MDI, a low-molecular weight aromatic diisocyanate which is commonly used to make polyurethane. MDI is also a major cause of occupational asthma, due to its ability to sensitize the immune system and elicit an immune response. In humans, the carrier protein albumin is able to recognise and conjugate with MID, forming a complex which is bound by IgE, triggering an asthmatic attack. Monoclonal antibodies directed against MID can be applied to diagnostics and research,and can be used to detect and quantitate MID levels in blood and urine, as well as in environmental samples. Monoclonal antibodies AD3 and AH6 bind to similar or overlapping epitopes.
Antibody first published in:
Wisnewski AV, Liu J. Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate. Mol Immunol. 2013 Jun;54(2):233-7. PMID:23295252
Note on publication:
Describes the production of six monoclonal antibodies directed against MDI, which could be developed into useful diagnostic reagents.