Anti-alpha-gal epitope [m86]

Name: Anti-alpha-gal epitope [m86]
SKU: Ab00532
Availability: InStock

Description:

Recombinant monoclonal antibody to alpha-gal epitope. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma m86.

Clone Name:

m86

Published Application(s):

ELISA, FC

Published Species Reactivity:

Species independent

Products based on this clone:

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Ab00532-10.0 Anti-alpha-gal epitope [m86]
Species independent
Human IgG1
Purified
In Stock

Ab00532-1.1 Anti-alpha-gal epitope [m86]
Species independent
Mouse IgG1
Purified
In Stock

Ab00532-15.0 Anti-alpha-gal epitope [m86]
Species independent
Human IgM
Purified
In Stock

Ab00532-14.0 Anti-alpha-gal epitope [m86]
Species independent
Human IgE
Purified
In Stock

Ab00532-21.0 Anti-alpha-gal epitope [m86]
Species independent
Mouse IgM
Purified
In Stock

Alternative Name(s) of Target: A-Gal; Gal-alpha-1 – 3Gal-beta-1 – 4GlcNAc-R
Immunogen: Rabbit red cells, on which α-gal is the major carbohydrate epitope, were injected into alpha 1,3 galactosyltransferase knock-out mice.

Specificity: This antibody binds to the alpha-gal epitope on glycoproteins and glycolipids.

Application Notes: This antibody binds to the alpha-gal epitope found on many glycoproteins and glycolipids of non-primate mammalis, prosimians and New World monkeys. Antibodies agains the alpha-gal epitopes occur naturally in Old World monkeys, apes and humans, and induce xenograft rejection from non-primate animals.

Antibody first published in: Chen, Radic and Galili Genes coding evolutionary novel anti-carbohydrate antibodies: studies on anti-Gal production in alpha 1,3 galactosyltransferase knock out mice. Mol Immunol. 2000 Jun;37(8):455-66. PMID:11090880
Note on publication: Describes the generation and characterization of anti-gal epitope antibodies raised in alpha 1,3 galactosyltransferase knock-out mice.

IF analysis of RAW264.7 cells using Ab00532-1.1. Click on image for more details.

Immunofluorescence staining of RAW264.7 cells using anti-alpha-gal epitope antibody (Ab00532) m86. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells stained with the chimeric mouse IgG version of m86 (Ab00532-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00532-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.

WB on rat spleen(A) and pig spleen(B) tissue lysate using Ab00532-1.1. Click on image for more details.

Western Blot using anti-alpha-gal epitope antibody m86 (Ab00532). rat spleen(A) and pig spleen(B) tissue lysate (35µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of m86 (Ab00532-1.1) at 0.3 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry analysis of RAW264.7 cells using Ab00532-1.1 Click on image for more details.

Flow cytometry using the Anti-alpha-gal epitope antibody m86 (Ab00532). Paraformaldehyde fixed RAW264.7 cells were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of m86 (Ab00532-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

FC using Ab00532-1.1 Click on image for more details.

Flow-cytometry using the anti-alpha-gal epitope antibody m86 (Ab00532). RAW264.7 cells were stained with anti-unknown IgG antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG-chimeric version of m86 (Ab00532-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

WB on rat spleen(A) and pig spleen(B) tissue lysate using Ab00532-1.1. Click on image for more details.

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