United Kingdom (UK)
Recombinant monoclonal antibody to CD45RB. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OX-33.
UniProt Accession Number of Target Protein: P04157
Alternative Name(s) of Target: L-CA; LCA; OX33; CD45 restricted; MRC OX33; MRC OX-33; CD45 R
Immunogen: Binds only to high MWT CD45RB. CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant).
Specificity: This antibody binds CD45RB and enables B cell (no other lymphoid cells) labelling analyzed by FACS. In addition it can be used fr immunofluorescent staining with FC analysis, IP and IHC of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
Application Notes: This antibody can be used for IP, FC, IHC and ICC
Antibody first published in:
Kato et al. CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation. J Exp Med 17:6-49 1992 PMID:1383383
Note on publication:
Describes the use of OX-78 in characterising CD48 as the ligand for CD2 in mice.
Western Blot using anti-CD45RB antibody OX-33 (Ab00555) Rat spleen lysate (35µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blot probed with the mouse IgG1 version of OX-33 (Ab00555-1.1) at 0.1 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow-cytometry using the anti-CD45RB antibody OX-33 (Ab00555) Rat splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the mouse IgG1 version of OX-33 (Ab00555-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.