United Kingdom (UK)
Recombinant monoclonal antibody to CD26. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OX-61.
UniProt Accession Number of Target Protein: P14740
Alternative Name(s) of Target: DPP4; DPP 4; DPPIV; DPP IV; Dipeptidyl peptidase 4; Bile canaliculus domain-specific membrane glycoprotein; GP110 glycoprotein; T-cell activation antigen CD26 ;OX61; MRC OX-61; MRC OX61
Immunogen: OX-61 was prepared by immunizing mice with rat thoracic duct lymphoid cells that were enriched for dendritic cells.
Specificity: OX-61 binds specifically to DPP4. Evidence: tissue distribution of OX-61 and DPP4 are identical; equal MWTs; OX-61-mediated reduction in DPP4 activity from solubilized liver homogenates. DPP4 is a glycoprotein receptor which acts as a positive regulator of T cell co-activation.
Application Notes: This antibody can be used for denaturing and non-denaturing WB and developed using peroxidase-conjugated rabbit anti-mouse. It can also be used for IHC of cryostat sections from adult rat liver. Staining is also observed on the brush border of the small intestine and colonic epithelium, T cell areas of lymphoid cells but not B cell follicles
Antibody first published in:
Paterson et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol 24:1281 1987 PMID:
Note on publication:
Describes the use of Ox-48 among other anitbodies to identify new markers for activated T-cells, which might have expression related to theactive Vs quiescient state or may be involved in specific T-cell functions.
Immunofluorescence staining of fixed rat splenocytes with anti-CD26 antibody OX-61 (Ab00568). Immunofluorescence analysis of paraformaldehyde fixed rat splenocytes on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OX-61 (Ab00568-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00568-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the anti-CD26 antibody OX-61 (Ab00568). Paraformaldehyde fixed rat splenocyteses were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of OX-61 (Ab00568-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.