United Kingdom (UK)
Recombinant monoclonal antibody to N-ethylmaleimide cysteine. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma OX-133.
Alternative Name(s) of Target: NEM cysteine; OX133
Immunogen: Purified MBP-gp120 (HIV-1) reduced using tris(2-carboxyethyl)phosphine (TCEP, 2.5 mM) for 20 minutes and alkylated using NEM (5 mM solubilized in PBS) for 30 minutes.
Specificity: This antibody recognises cysteine-boud N-ethylmaleimide in a sequence-independent manner. It has no affinity for unmodified peptides or peptides modified with other alkylating agents such as iodoacetamide.
Application Notes: OX133 detects NEM-modified proteins and can be used as an affinity matrix to purify NEM-modified proteins from cell lysates. OX133 does not cross react with any other alkylating agent, making it a highly selective reagent for the purification of NEM-labeled proteins and potentially peptides for mass spectrometry-based analysis.
Antibody first published in:
Holbrook et al. OX133, a monoclonal antibody recognizing protein-bound N-ethylmaleimide for the identification of reduced disulfide bonds in proteins MAbs. 2016 May-Jun;8(4):672-7 PMID:26986548
Note on publication:
Describes the generation and characterisation of this antibody.