Anti-MHC II [YKIX 334.2] Standard size

Description: Recombinant monoclonal antibody to MHC II. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma YKIX 334.2. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Isotype and Format: Rat IgG2a, Fc Silent™, kappa
Clone Number: YKIX 334.2
Published Application(s): FC; IP; block, IHC
Published Species Reactivity: Dog
Immunogen: YKIX 334.2 was prepared by immunizing rats with concanavilin A activated canine peripheral blood cells.
Specificity: YKIX 334.2 binds specifically to the MHC II beta chain. In dogs, MHC Class II is expressed on the surface of all peripheral blood mononuclear cells where it has a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells, macrophages).
Application Notes: YKIX 334.2 immunoprecipitates an antigen of 32/34kD and can has been shown to block the proliferation of MHC Class II dependent responses in vitro. This antibody can also be used for IHC-Fr and FC.
Antibody First Published in:
Cobbold et al. Tissue Antigens. 1994 Mar;43(3):137-54. PMID:8091414 Note on publication: Describes the binding specificity and characterisation (by IF staining and FACS, and IP) of antibodies against canine equivalents of human CD antigens.

Product Form

This is a Standard size product. Purified antibody.
Purification: Protein A affinity purified
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00600-7.4

Rat IgG2a

Ships in 2-3 weeks
Standard size 200 µg
Custom conjugation:
Clear selection
Ships in 2-3 weeks
Bulk size
1 mg