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- Ab00604-2.0 Anti-CD45RO [UCH-L1]
- Human
- Mouse IgG2a
- Purified
- In Stock
- Ab00604-2.3 Anti-CD45RO [UCH-L1]
- Human
- Mouse IgG2a
- Fc Silent™
- Purified
- In Stock
- Ab00604-23.0 Anti-CD45RO [UCH-L1]
- Human
- Rabbit IgG
- Purified
- In Stock
Recombinant monoclonal antibody to CD45RO. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma UCH-L1.
Alternative Name(s) of Target: PTPRC; Protein tyrosine phosphatase; receptor type C; CD45 R; UCHL1; UCHL-1
Immunogen: Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.
Specificity: This antibody binds human CD45RO.
Application Notes: PTPRC (CD45) is a transmembrane tyrosine phosphatase that is present on all leukocytes. PTPRC regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). CD45R and CD45RO are two of multiple PTPRC isoforms generated by alternative splicing. CD45R is found on B lymphocyte and T lymphocyte subsets. CD45RO is present on 50% of T cells, most granulocytes and monocytes and most mature T cell tumours. This antibody reacts with an epitope unique for CD45R0. It may identify abnormalities of functional T-cell subsets in immune disorders, be used in tumour diagnosis by FACS or immunohistology and has been very useful in studies of human T cell function and ontogeny.
Antibody first published in: PMID:
Flow-cytometry using anti-CD45RO antibody UCH-L1 (Ab00604) Human leukocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of UCH-L1 (Ab00604-23.0, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.