Anti-Integrin beta-7 [FIB27] Bulk size

Description: Recombinant monoclonal antibody to Integrin beta-7. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma FIB27. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors.
Isotype and Format: Rat IgG2a, Fc Silent™, kappa
Clone Number: FIB27
Published Application(s): FC; IP; block
Published Species Reactivity: Human; Mouse
Immunogen: FIB27 was prepared by immunizing rats with TK1 cells.
Specificity: FIB27 binds specifically to both human and mouse integrin beta-7 at epitope region Dl on which is involved in all a4b7-mediated adhesion events. Does not compete with DAKT32 for binding. FIB27 blocks LS722-induced TK1 cell aggregation (mAb LS722 is an activating anti-a4B7 antibody and induces aggregation via an a4B7-dependent pathway). Integrin beta-7 is one subunit is a heterodimer which makes up an intergin molecule. In the mouse beta-7 is selectively found on the majority of mature lymphocytes, whereas a small subpopulation of thymocytes and bone marrow cells express beta-7. Beta-7 is also expressed in variable amounts in CD4 T memory cells. Integrin molecules mediate cell-cell and cell-extracellular matrix adhesion and are involved in lymphocyte homing, leukocyte recruitment to inflammatory sites, myogenesis, hemopoiesis, and melanoma metastasis.
Application Notes: FIB27 can be used for FC, IP and can block TKI cell aggregation and beta-7 intergrin-mediated cell adhesion.
Antibody First Published in:
Andrew DP, et al. J. Immunol. 153:3847 1994 PMID:7523506 Note on publication: Describes the use of FIB27 in defining functional binding regions of alpha-4/beta-7 integrin by epitope mapping.

Product Form

This is a Bulk size product. Purified antibody in bulk size.
Purification: Protein A affinity purified
Supplied in: PBS only.
Storage Recommendation: Store at 4°C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00627-7.4-BT

Rat IgG2a

Ships in 2-3 weeks
Bulk size
1 mg

£1,160

Ships in 2-3 weeks
Standard size 200 µg
Custom conjugation:
Clear selection