Anti-CD44 [Hermes-1] Bulk size

Description: Recombinant monoclonal antibody to CD44. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Hermes-1.
Isotype and Format: Human IgG1, kappa
Clone Number: Hermes-1
Published Application(s): Blocking; ELISA; FC; ICC/IF; IHC; IP; Neut; WB
Published Species Reactivity: Human, Mouse, Rat, Goat, Primate, Rabbit, Sheep, Bovine
Immunogen: This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).
Specificity: This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44.
Application Notes: This antibody binds CD44, so can be used to label CD44+ cells in flow cytometry analysis. As CD44 molecules are used to bind to high endothelial venules (HEV) at the site of lymphoctye entry to lymphoid organs, this antibody can be used to specifically stain HEV-binding cell lines (Jalkanen, 1986). In addition, this antibody blocks the adhesion of the haematopoietic progenitor cell line KG1 to hyaluronic acid (Bendall, 2003), but does not block lymphocyte binding to HEV (Picker, 1989). 7H9D6 partially blocks the binding of this antibody.
Antibody First Published in:
Jalkanen et al. Eur J Immunol. 1986 Oct;16(10):1195-202. PMID:2429846 Note on publication: Describes the original use of this antibody in preliminary attempts to characterise its ligand (CD44), using IHC-fr, IP and IF.

Product Form

This is a Bulk size product. Purified antibody in bulk size.
Purification: Protein A affinity purified
Supplied in: PBS only.
Storage Recommendation: Store at 4°C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00628-10.0-BT

Human IgG1

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Standard size 200 µg
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