United Kingdom (UK)
Recombinant monoclonal antibody to Bet v 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the single-chain antibody M0418.
UniProt Accession Number of Target Protein: P15494
Alternative Name(s) of Target: Major Allergen from Birch Pollen; BETVIA
Immunogen: Bet v 1-A, a major allergen of the European white birch.
Specificity: This antibody binds to Bet v 1, in particular to the naturally occurring isoforms Bet v 1.0101 and Bet v 1.0102, but not to Bet v 1.0112.
Application Notes: This antibody binds to Bet v 1 via a charaterized epitope and has been used in crystallographic studies.
Antibody first published in:
Levin et al. Human IgE against the major allergen Bet v 1 – defining an epitope with limited cross-reactivity between different PR-10 family proteins Clin Exp Allergy. 2014 Feb;44(2):288-99. PMID:24447087
Note on publication:
Describes the generation and characterization of the M0418 antibody.
Binding curve of anti-Bet v 1 antibody M0418 (Ab00649-23.0) to Bet v 1 ELISA Plate coated with Bet v 1-A (RayBiotech, 228-22389) at a concentration of 2 µg/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using Ab00649-23.0. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more, as also observed for Ab00648-23.0. This is suggestive of two binding sites on Bet v 1-A for which Ab00649-23.0 has differing affinities.