United Kingdom (UK)
Recombinant monoclonal antibody to Bla g 2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4C3.
UniProt Accession Number of Target Protein: P54958
Alternative Name(s) of Target: Cockroach Allergen; Aspartic protease Bla g 2; Blattella germanica allergen
Immunogen: Bla g 2, a major cockroach allergen.
Specificity: This antibody binds to the C-terminal lobe of Bla G 2, via an epitope containing a carbohydrate moiety (on Asn268, however non-essential for binding, though a 40-fold reduction in affinity is observed).
Application Notes: This antibody binds to Bla g 2 via an epitope distinct from the epitope used by anti-Bla g 2 antibody clone 7C11 also available through Absolute Antibody. Both antibodies may be used in a sandwich ELISA.
Antibody first published in:
Li et al. Carbohydrates contribute to the interactions between cockroach allergen Bla g 2 and a monoclonal antibody J Immunol. 2011 Jan 1; 186(1): 333–340. PMID:21123808
Note on publication:
Describes the generation and use of this antibody in a co-crystallization study as well as in a sandwich ELISA with clone 7C11