United Kingdom (UK)
Recombinant monoclonal antibody to CD37. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MB-1.
UniProt Accession Number of Target Protein: P11049
Alternative Name(s) of Target: Tetraspanin-26; Tspan-26; Leukocyte antigen CD37;
Immunogen: BALB/c mice were immunized with lymph node cells from a patient with nodular poorly differentiated lymphocytic lymphoma. Immunophenotyping studies of these cells identified them as B cells expressing SIgG/λ, as well as HLA-DR. Leu-12, and B1.
Specificity: This antibody is specific for human CD37, which is expressed on virtually all mature B lymphocytes, but not on pro-B or plasma cells. CD37 is highly expressed on all forms of B cell non-Hodgkin lymphoma, chronic lymphocyte leukemia, and other leukemias. CD37 does not appear to be expressed on T cells, thymocytes, granulocytes or platelets.
Application Notes: This antibody recognizes human CD37, and has been used to identify and characterise the CD37 antigen in cell labelling, indirect immunofluorescence and IP analysis (Link, 1986). In particular, the use of this antibody to label malignant B cells indicates that expression of CD37 occurs relatively late in B cell maturation, as it is not expressed on "immature" B lineage lymphoid neoplasms.
Antibody first published in:
Link et al. A unique antigen on mature B cells defined by a monoclonal antibody. J Immunol. 1986 Nov 1;137(9):3013-8. PMID:3489782
Note on publication:
Describes the original use of this antibody to identify a novel B cell antigen (CD37)
Immunofluorescence staining of Daudi cells using anti-CD6 (Ab00698) OX-126 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells stained with the chimeric mouse IgG version of OX-126 (Ab00698-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00698-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-CD37 antibody MB-1 (Ab00698). Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of MB-1 (Ab00698-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.