United Kingdom (UK)
Recombinant monoclonal antibody to IgE. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody huMaE11 (Omalizumab).
UniProt Accession Number of Target Protein: P01854
Alternative Name(s) of Target: epsilon chain; Ig epsilon chain C region; immunoglobulin E
Immunogen: Human IgE protein.
Specificity: This antibody binds to circulating human IgE antibody, but not to cell-bound IgE. It binds in proximity of the binding site of the high-affinity IgE receptor Fc epsilon RI.
Application Notes: This antibody binds to circulating IgE, thereby inhibiting IgE binding to mast-cells. It does not bind to cell-bound IgE and does not provoke histamine release from mast-cells sensitised by IgE. As a human IgG1, marketed as Xolair™, it is used therapeutically to treat asthma and allergies.
Antibody first published in:
Presta et al. Humanization of an antibody directed against IgE. J Immunol. 1993 Sep 1;151(5):2623-32. PMID:8360482
Note on publication:
Describes the humanization of the original murine antibody MaE11.
Binding curve of anti-IgE antibody huMaE11 (Omalizumab) (Ab00717-10.0) to human IgE Fc domain ELISA Plate coated with human IgE Fc domain (Pr00104-14.5) at a concentration of 5 µg/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using Ab00717-10.0. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.