United Kingdom (UK)
Recombinant monoclonal antibody to EGFR domain III. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the therapeutic antibody h-R3 (Nimotuzumab).
UniProt Accession Number of Target Protein: P00533
Alternative Name(s) of Target: ERBB-D3; ERBB1; HER1; PIG61; mENA; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; epidermal growth factor
Immunogen: This antibody we generated by humanisation of the murine antibody R3, raised against EGFR purified from placenta membranes and A431 tumors.
Specificity: Binds to human EGFR domain III at a site distinct from antibody 7A7 (Ab00134) (Talavera et al. 2011)
Application Notes: This antibody binds to human EGFR and inhibits EGF-dependent clonogenic growth of NRK cells and completely blocks EGF stimulated autophosphorylation of the receptor (Fernandez 1992)
Antibody first published in:
Fernandez et al. A new monoclonal antibody for detection of EGF-receptors in western blots and paraffin-embedded tissue sections. J Cell Biochem. 1992 Jun;49(2):157-65. PMID:1400622
Note on publication:
Describes the generation and characterisation of the R3 antibody from which h-R3 was generated by humanisation.
Immunofluorescence staining of fixed HeLa cells with anti-EGFR domain III antibody h-R3 (Nimotuzumab) (Ab00723) Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit version of h-R3 (Ab00723-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00723-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor® 488 secondary antibody.