Anti-HBeAg [E6] Standard size

Description: Recombinant monoclonal antibody to HBeAg. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma E6. This is a Fab fragment with a his-tag.
Isotype and Format: Mouse Fab fragment, His-Tagged, kappa
Clone Number: E6
Published Application(s): Mab: RIA; cross-competition assay; serum protein electrophoresis. Fab; crystallization
Published Species Reactivity: Hep B
Immunogen: This antibody was produced by immunizing BALB/c and SJL/Olac mice with HBeAg derived by denaturing human liver HBcAg. The subsequent production of the Fab is not detailled.
Specificity: This antibody defines the e-antigen determinant HBe-β, and is specific for unassembled dimers. Two copies of the antibody are able to bind captured HBeAg dimers, indicating two accessible epitopes per dimer. E6 binding is inhibited by other members of the e-β-reactive group of Mabs, namely e4, e8 and e9.
Application Notes: This Fab forms a stable complex with the recombinantly-expressed HBeAg dimer. Consequently, this antibody can be used to facilitate the crystallization of the complex, and the establishment of its structure (DiMattia, 2013).
Antibody First Published in:
Ferns et al. The Journal of general virology, May 1984, Vol.65 ( Pt 5), pp.899-908 PMID:6202830 Note on publication: Ferns et al (1984) descibes the original production of murine e6, along with a panel of other anti-HBeAg Mabs, for use in the characterisation of HBeAg.

Product Form

This is a Standard size product. Purified antibody.
Purification: Purified by Immobilized Metal Affinity Chromatography
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00732-1.6

Mouse Fab fragment

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Standard size 200 µg
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