United Kingdom (UK)
Recombinant monoclonal antibody to Prion protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma VRQ14.
UniProt Accession Number of Target Protein: P23907
Alternative Name(s) of Target: CD230; PrP scrapie-senstive variant VRQ; PrP; major prion protein
Immunogen: The VRQ14 antibody was prepared by immunizing Prnp0/0 mice with the C-terminal domain of recombinant ovine VRQ variant prion protein. The Fab fragemnt was produced by digestion with papain.
Specificity: VRW14 binds to both PrPC (host prion protein) and to PrPSc (pathogenic isoform of the prion protein) - the epitope of the antibody mainly consists of PrP residues 188–199 (C-terminal part of helix H2 and N-terminal part of the H2–H3 loop). Misfolding and aggregation of mutated protein PrP leads to prion diseases which are associated with the conversion of the α-helix rich prion protein (PrPC) into a β-structure-rich insoluble conformer (PrPSc). Prion diseases are neurodegenerative pathologies which affect numerous mammalian species and different mutations in the PRNP gene result in protein products with different susceptibilities to causing disease.
Application Notes: VRQ14 can be used to facilitate the crystallisation of ovine PrP in addition to being used in (sandwich) ELISA and IP studies.
Antibody first published in:
Eghiaian et al. Insight into the PrPC-->PrPSc conversion from the structures of antibody-bound ovine prion scrapie-susceptibility variants. Proc Natl Acad Sci U S A. 2004 Jul 13;101(28):10254-9. Epub 2004 Jul 6. PMID:15240887
Note on publication:
Describes the use of VRQ14 in helping to deduce the PrPC to PrPSc transition and to aid the crystallisation of PrP.