Anti-Fc gamma receptor III [3G8] Standard size

Description: Recombinant monoclonal antibody to Fc gamma receptor III . Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3G8.
Isotype and Format: Human IgG1,
Clone Number: 3G8
Published Application(s): FC; SPR; depletion; IP; IHC; block
Published Species Reactivity: human; chimpanzee; baboon; Cynomolgus; Rhesus; Pigtailed Macaque; Capuchin Monkey; Squirrel Monkey; Sooty Mangabey; Cotton-topped Tamarin; Marmoset
Immunogen: 3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.
Specificity: 3G8 binds to CD16 at an epitope which is not the IgG-binding site. This antibody cannot distinguish between CD16a transmembrane isoform found on the surface of NK cells and macrophages (which can trigger the cytolytic response), and the GPI-linked CD16b isoform present on neutrophils or soluble CD16 found in the plasma. CD16 is the low affinity IgG receptor III and binds to aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis and ADCC (antibody-dependent cell-mediated cytotoxicity).
Application Notes: 3G8 antibody was used to construct a recombinant bispecific scFv (bsscFv) along with the mAb clone F3.3 anti-HLA class II antibody (connected by a (Gly4Ser)4 linker)which was expressed in SF21 insect cells. This bsscFv allows NK cells to target malignant B lymphoid cells and mediates effector cell lysis (ASCC) of the malignant cells (also lysis of primary leukaemia samples). 3G8 is also able to block neutrophil phagocytosis and stimulates NK cell proliferation, and can be used in FC, SPR, IP and IHC studies.
Antibody First Published in:
Bruenke et al. Br J Haematol. 2004 Apr;125(2):167-79. PMID:15059139 Note on publication: Describes the construction and characterisation of recombinant bsscFv capable of targetting CD16+ immune cells to maligant B cells resulting in ADCC.

Product Form

This is a Standard size product. Purified antibody.
Purification: Protein A affinity purified
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label

Product Code: Ab00754-10.0

Human IgG1

Ships in 2-3 weeks
Standard size 200 µg
Custom conjugation:
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