United Kingdom (UK)
Recombinant monoclonal antibody to Trimeric Lewis X. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 54-5C10-A.
UniProt Accession Number of Target Protein: O02197
Alternative Name(s) of Target: LeX; Lewis X; LewisX; trimeric LeX; Galbeta1-4(Fucalpha1-3)GlcNAcbeta; Lewis X trisaccharide
Immunogen: 54-5C10-A mAb was prepared from generating a hybridoma cell line derived from lymphocytes from mice with a schistosomal infection. The mAb was selected for by screening for interaction with the Lewis X epitope by SPR.
Specificity: 54-5C10-A Fab requires at least three repeating units of the Lewis X trisaccharide to bind to CCA (circulating cathodic antigen - an antigen released from the gut of the adult worm which contains different oligomeric presenations of Lewis X). CCA is thought to have an important role in modulating the host's immune response. Lewis X is also found one of the carbohydrate blood group antigens and plays a role in cell adhesion by both carbohydrate-carbohydrate and carbohydrate-protein interactions.
Application Notes: 54-5C10-A can be used in IF assays and can be crystallised as a Fab fragment. The antibody can also be used for SPR and in ELISA (allows diognosis schistosomiasis in humans).
Antibody first published in:
de Geus et al. Characterization of a diagnostic Fab fragment binding trimeric Lewis X. Proteins. 2009 Aug 1;76(2):439-47. PMID:19173313
Note on publication:
Describes the generation of 54-5C10-A and its characteriztion, including its binding affinity and specificity by SPR, IF assays, structure determination of the Fab by Xray crystallography and its mode of binding to Lewis X via docking.