United Kingdom (UK)
Recombinant monoclonal antibody to Junin virus GP1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma GD01.
UniProt Accession Number of Target Protein: P26313
Alternative Name(s) of Target: JUNV; GP1; JUNV GP1; GPC; Glycoprotein G1; Pre-glycoprotein polyprotein GP complex; Argentinian mammarenavirus; glycoprotein receptor-binding subunit 1; surface envelope glycoprotein; Pre-GP-C; GD01-AG02; J200
Immunogen: The original antibody was generated by immunizing BALB/c mice with inactivated-virus-infected BALB/c suckling mouse brain emulsion.
Specificity: This antibody is specific for the GP1 subunit of the JUNV surface envelope glycoprotein GPC.
Application Notes: The original version of this antibody (mouse IgG2a) successfully bound Junin virus (XJ45 strain) GP1 in an ELISA. This antibody was used for the indirect immunofluorescent staining of Junin virus-infected Vero cells. This antibody reacted with Junin GP1 in immunoprecipitation and western blot. This antibody successfully neutralized the Junin virus in an in vitro assay (Sanchez et al., 1989; PMID: 2471803). The GP1-bound Fab version of this antibody was crystallized, and its structure was determined. This antibody neutralized the active form of JUNV by disrupting the interaction between GP1 and TfR1. A competition assay showed that IgGs from survivors of AHF did not compete with this antibody (Mahmutovic et al., 2015; PMID: 26651946). This antibody exhibited potent neutralization of the Candid #1 JUNV strain in a plaque neutralization assay (Zeitlin et al., 2016; PMID: 27044104).
Antibody first published in:
Sanchez et al. Junin virus monoclonal antibodies: characterization and cross-reactivity with other arenaviruses. J Gen Virol. 1989 May;70 ( Pt 5):1125-32. PMID:2471803
Note on publication:
The original publication explores the production, characterization, and cross-reactivity of monoclonal antibodies targeting Junin virus structural proteins, revealing insights into their interaction with other arenaviruses.