Anti-single-stranded poly(rI) RNA [Jel 103] Standard size

Description: Recombinant monoclonal antibody to single-stranded poly(rI) RNA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Jel 103.
Isotype and Format: Rabbit IgG, kappa
Clone Number: Jel 103
Published Application(s): SPRIA; IF
Published Species Reactivity:
Immunogen: Jel 103 was selected from a hybridoma cell line generated by the fusion of the MOPC 315-43 myeloma cell line with mouse spleen cells derived from C57/B1 mice which had been immunized with poly(rI)poly(dC) duplex complexed to methylated bovine serum albumin.
Specificity: Jel 103 is highly specific for single-stranded RNA and shows sequence specificity as it binds to poly(rI) but not to poly(dI), poly(rG) or to any other homopolymer - it does not bind randomly to calf thymus DNA. Jel 103 Fab is shown to form base stacking interactions with a Tyr residue and two salt-bridge interactions to the phosphate backbone with Lys and Arg residues.
Application Notes: The Jel 103 Fab fragment is generated from papain digestion. Jel 301 can be used for the detection of single-stranded poly(rI) and for binding studies where the antibody has been used previosly in solid-phase radioimmunoassays.
Antibody First Published in:
Pokkuluri et al. J Mol Biol. 1994 Oct 21;243(2):283-97. PMID:7523684 Note on publication: Describes the generation of jel 301 fab fragment and the structures of the fab in complex with rIP, rGDP and dIMP are reported.

Product Form

This is a Standard size product. Purified antibody.
Purification: Protein A affinity purified
Supplied in: PBS with 0.02% Proclin 300.
Storage Recommendation: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Concentration: See vial label